Avoid Boring People: Lessons from a Life in Science

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Avoid Boring People: Lessons from a Life in Science Page 11

by James D. Watson


  I was by then having lunch with Francis almost daily at a nearby pub, the Eagle, which during the war was favored by American airmen flying out of nearby airfields. Soon we would be upgraded from desks beside our lab benches to a largish office of our own next to the connected pair of smaller rooms used by Max and John. In this way, Francis's ever irrepressible laughter would less disturb the work habits of other unit members. At our first meeting, Francis had spoken of his much valued friend Maurice Wilkins, who, like him, had made a wartime marriage that soon disintegrated with peace. Because he was curious to know whether Maurice's crystallography had generated any new, perhaps sharper X-ray photos from DNA, Francis invited him for a Sunday dinner at the Green Door, the tiny apartment on top of a tobacconist's on Thompson Lane, across from St. John's College. Earlier occupied by Max Perutz and his wife, Gisela, it had been home to Francis and his second wife, Odile, since their marriage two years before, in August 1949.

  At that meal, we learned of an unexpected complication to Maurice's pursuit of DNA. While he was on an extended winter visit to the United States, his boss, J. T. Randall, had recruited to the King's DNA effort the Cambridge-trained physical chemist Rosalind Franklin. For the past four years in Paris she had been using X-rays to investigate the properties of carbon. Rosalind understood from Randall's description of her responsibilities that X-ray analysis of DNA was to be her responsibility solely. This effectively blocked Maurice's further X-ray pursuit of his crystalline DNA. Though not formally trained as a crys-tallographer, Maurice had already mastered many procedures and had much to offer. But Rosalind didn't want a collaborator; all she wanted from Maurice was the help of his research student Raymond Gosling. Now, though out in the cold for two months, Maurice could not stop thinking about DNA. He believed his past X-ray pattern arose not from single polynucleotide chains but from helical assemblies of either two or three intertwined chains bonded to each other in a fashion as yet to be determined. With the DNA ball sadly no longer under his control, Maurice suggested that if Francis and I wanted to learn more we should go to King's in a month's time to hear Rosalind give a talk on November 21.

  Rosalind Franklin, while in Paris, serving afternoon coffee in evaporating dishes

  Before it was time to go to London, Francis had reason to feel good about his place in the Cavendish. He and the clever crystallographer Bill Cochran derived easy-to-use mathematical equations for how helical molecules diffract X-rays. Each of them, in fact, did so independently within twenty-four hours of being shown by Bragg a manuscript from Vladimir Vand in Glasgow, whose equations they immediately saw as only half-baked. Theirs was an important achievement, for Francis and Bill had given the world the equations that could predict the diffraction patterns of specific helical molecules. The next spring I was to deploy them to show that the protein subunits of tobacco mosaic virus are helically arranged.

  Suddenly, the best way to reveal DNA's 3-D structure was to build molecular models using Cochran and Crick's equations. Until a year previously this approach had made no sense since the nature of the covalent bonds linking nucleotides to each other in DNA chains was unknown. But after work by Alex Todd's nearby research group in the chemical laboratory at Cambridge, it was clear that DNA's nucleotides are held together by 3'-to-5’ phosphodiester bonds. A focus on model building was a way to set oneself apart from the alternative approach of focusing on X-ray photograph details being pursued at King's College in London.

  On the day of the lecture, Francis was unable to go down to London and I went alone, still oblivious to the difference between the crystallographic terms “asymmetric unit” and “unit cell.” As a result, the next morning I mistakenly reported to Francis that Rosalind's DNA fibers contained very little water. My error only came to light a week later, when Rosalind and Maurice came up from London to look at a three-chain model that we had hastily constructed. It had DNA's sugar-phosphate backbone in the center with the bases facing outward. Upon seeing it, Rosalind immediately faulted its conception, saying the phosphate groups were located on the outside, not the inside of the molecule. Moreover, we had proposed DNA to be virtually dry whereas, in fact, it was highly hydrated. And we got the unmistakable impression that the King's group considered the pursuit of the DNA structure to be their property, not one to be shared with their fellow MRC unit in Cambridge. All too soon we learned that Sir Lawrence Bragg was of the same mind, when he told us to refrain from all subsequent DNA model-building activities. In stopping us Bragg was not motivated solely by a need to remain on good terms with another MRC-supported group. He wanted Francis to focus exclusively on research for his Ph.D. and be done with it.

  This debacle, however, would not have occurred if Francis and I had started to think as if we were chemists. Even without the King's X-rays, there were sufficient clues in the chemical literature that should have led us to propose a double helix as the basic structure of DNA. From the start we should have restricted ourselves to models in which externally located sugar-phosphate backbones were held together by hydrogen bonds between centrally located bases. Strong physical chemical evidence for bases so held together had come from the postwar experiments of John Gulland. In 1946, his Nottingham lab showed that within native DNA molecules the bases are so arranged as to hinder them from exchanging hydrogen atoms. These data suggested widespread hydrogen bonding between DNA bases. This insight was widely available, published by Cambridge University Press in the 1947 Society for Experimental Biology symposium volume on nucleic acids.

  Furthermore, given Linus Pauling and Max Delbrück's prewar proposal that the copying of genetic molecules would involve structures of complementary shape, Francis and I should have reasonably focused on two-chain rather than three-chain models. Thinking this way, each DNA base should hydrogen-bond exclusively to one with a molecule of complementary shape. In fact, experimental data pointing to this conclusion, too, already had been published, most coming from the lab of the Austrian-born chemist Erwin Chargaff in New York. Without understanding the significance, he reported that in DNA the amounts of the purine adenine were roughly equal to the amount of the pyrimidine thymine. Likewise, the amount of the second pur ine, guanine, was similar to the amount of the second pyrimidine, cytosine.

  The exact shape of such base pairs would depend upon where the atoms available for hydrogen bonding were located on each base. In 1951, few chemists knew enough quantum mechanics to make such inferences. So that fall we should have sought advice from the several British chemists trained in this esoteric field. In retrospect, Alex Todd's lab, after determining the covalent linkages in DNA, should have moved on to determining what the molecule looks like in three dimensions. But in those days, even the best organic chemists thought such problems were better left to X-ray crystallographers. In turn, most X-ray diffraction experts felt the time had not yet arrived to tackle biological macromolecules. In a sense, then, the field was wide open.

  Even after he found the a-helix, Linus Pauling remained only moderately attentive to DNA, never seriously believing then that it had a genetic role. Even so, when hearing of Maurice Wilkins's crystalline photo, he asked to have a look, being misinformed that Maurice himself was not seriously trying to determine the structure. As that was precisely what Maurice was up to, he quickly replied that he wanted more time to look over the photo before releasing it to others. Undeterred, Linus wrote directly to the King's boss, John Randall, but this approach was likewise unsuccessful. Linus lost the scent until a year later at a summer phage meeting outside Paris, where he first learned of the work recently completed at Cold Spring Harbor by Alfred Hershey and Martha Chase, showing phage genes were also made from DNA. The news convinced Linus he must go after the DNA structure despite his lack of high-quality DNA X-ray photos. His voyage back to the States could have been a fortuitous opportunity. Also on board the transatlantic boat was Erwin Chargaff, who like Pauling had come to Europe to attend that summer's International Biochemical Congress in Paris. But instead of learning ab
out the equivalence of A with T and G with C, Linus took an instantaneous dislike to his shipmate and avoided him all across the Atlantic.

  Alfred Hershey's group at Cold Spring Harbor, 1952: Niccolò Visconti, Martha Chase, AlHershey, Constance Chadwick, Neville Symonds, June Dixon, and Alan Garen

  Preoccupied much of the fall of 1952 with the race against Francis Crick for the coiled coil structure of α-keratin, Pauling turned to DNA in earnest only in late November. Soon he was very much attracted to a DNA model in which three sugar-phosphate backbones coiled around each other. He was hung up on three chains because of the reported high density of DNA. At no time did he seriously consider a two-chain molecule. To hold the three chains together, he conceived of DNA as uncharged, forming hydrogen bonds between opposing phosphate groups. Soon satisfied that he had found the general structure for nucleic acids, he wrote to Alex Todd a week before Christmas, adding he was not bothered that his structure provided no clues as to how DNA functions in cells. That problem was for another day. At no time did he ever take into account Chargaff's base compositions, published more than a year before in several journals. The essential parameters for Linus that December were bond angles and length, not what DNA did biologically or how it behaved in solution. It was immediately evident that the atoms of his model were not fitting together as neatly as they did in the a-helix. Even his best structure was stereo-chemically shaky, with several central phosphate oxygens uncomfortably close to each other.

  Fearing that someone in England might beat him to the punch with a similar model, Linus hastily submitted a manuscript for publication in the Proceedings of the National Academy. Then he triumphantly sent two manuscript copies to Cambridge—one to Bragg, the other to his son Peter. We were instantly engulfed in anxiety until we realized that Linus had used hydrogen atoms belonging to the phosphate groups to hydrogen-bond the three chains together. We knew at once his model must be wrong since DNA—an acid—normally releases all its hydrogen ions in solution, and so Francis and I rushed around Cambridge to see whether the local chemical hotshots also found Pauling's concept totally implausible. Quickly reassured by Alex Todd that Linus had indeed made a gigantic chemical goof, I went down almost immediately to London to show the manuscript to Maurice Wilkins and Rosalind Franklin, the latter preparing to move to J. D. Bernal's group in Birkbeck College, where she would no longer work on DNA.

  Maurice was more than relieved to learn that Linus was so far off base. In contrast, Rosalind was annoyed at my showing her the manuscript, tartly telling me that she had no need to read about helices. In her mind, the crystalline DNA A-form structure was most certainly not helical. In fact, six months before, she had sent out invitations to a July “memorial service” to celebrate the death of the DNA helix. Here Maurice thought that Rosalind had been badly deluding herself, and to prove it he impulsively showed me an X-ray photo that the King's group had been keeping secret since Raymond Gosling took it more than nine months before. Originating from a more hydrated B-form DNA fiber, this picture displayed unequivocally the large cross-shaped diffraction pattern to be expected from a helical molecule. My jaw dropped, and I rushed back to Cambridge to tell everyone what I had learned. In my mind we should not wait a moment longer before commencing to build models. Someone was bound to tell Linus that his three-chain model was dead on arrival. Sir Lawrence Bragg instantly agreed, and with him finally behind us Francis and I soon were back playing with cutout shapes. By then I realized that DNA's density did not, as I originally thought, rule out two strands as opposed to three. It thus made sense for me to focus first on possible ways for two DNA chains to twist around each other.

  In fact, Rosalind also should have been focusing on two-chain DNA models. More than a year before, she had carefully measured her X-ray diffraction patterns from crystalline A-form DNA, looking for possible molecular symmetries. Finding her data compatible with three possible chemical “space groups,” she went up to Oxford to get advice from Dorothy Hodgkin, then England's premier crystallographer, justly famed for solving the problem of the structure of penicillin. As soon as Dorothy saw that Rosalind was considering space groups involving mirror symmetry, however, she sensed crystallographic cal-lowness. Experienced crystallographers would never postulate mirror symmetry for a molecule made up exclusively of 2-deoxy-D-ribose. Instead, Dorothy believed, Rosalind should now have been considering only the implications of the third monoclinic space group (a rectangular prism of three unequal axes). Upset by Dorothy's sharp putdown of her crystallographic acumen, Rosalind left Oxford, never to return. If she had gone instead to Francis for help, she would have immediately learned that the C2 monoclinic space group suggested that DNA was a double helix with its chains running in opposite directions.

  Francis learned of DNA's monoclinic space group only through reading a nonconfidential King's progress report sent to Max Perutz in mid-February. By then, through a new burst of model building, I had found that a sugar-phosphate backbone of 20 Ä diameter optimally repeats every 34 Ä, the repeat distance measured in B-form DNA. Francis now argued, in light of Rosalind's space group, that the two chains must run in opposite directions. But I didn't initially buy this assertion, not understanding the underlying crystallographic symmetry argument. Until I knew how the centrally located bases bonded to each other, I didn't want to worry about backbone directions. Then, unknown to me, my model building was being hindered by faulty textbook descriptions for the structures of guanine and thymine. Using such false configurations, I had become momentarily excited about a pairing scheme similar to that found in crystals of adenine.

  That scheme, however, would have given a 17 Ä repeat along the helical axis, not the 34 Ä figure observed by Rosalind. Happily, the Cal-tech structural chemist Jerry Donohue, then spending his sabbatical year in Cambridge, set me on the right track by arguing that the guanine and thymine hydrogens should have keto rather than the textbook-ascribed enol configurations. Needing only a day to incorporate Jerry's reasoning, I changed the locations of the hydrogen atoms on my paper-cutout models of thymine and guanine. Almost instantly I found myself forming the A-T and G-C base pairs we now know to exist in DNA. Coming a half hour later into our office that Saturday morning, Francis took only a few minutes to conclude that symmetry of the base pairs demanded that the chains run in opposite directions. Rosalind's monoclinic space group was in a true sense a prediction of a model derived by Francis and me from purely stereochemical arguments. The double helix had to be correct. All that remained to be done was to build a backbone segment and measure its atomic coordinates to show that all the bond lengths and angles in our model agreed with those previously found in smaller molecules. This task, which for the first time in months took Francis away from his desk, took less than three days to complete. The double helix was ready to be let loose upon the world.

  Breaking the news to Wilkins that we very likely had solved the DNA structure was bound to cause his heart to spasm. A day after we had verified appropriate coordinates for all the atoms, a letter from him arrived informing Francis that Rosalind was out of King's and that Maurice was about to resume work on DNA. Perhaps to soften the blow, John Kendrew, not Francis, called Maurice to report that Francis and I had a promising novel structure for DNA. Coming up the next day, Maurice instantly recognized the double helices’ elegant simplicity and agreed that it was likely too good not to be true. Aware that we would not have found the DNA structure without knowledge of X-ray results from King's, Francis and I suggested to Maurice that his name also be on the manuscript we planned to send to Nature. Without hesitation he declined, possibly not knowing how to deal with Rosalind Franklin and Raymond Gosling's equally important contributions. The April 25,1953, issue of Nature, besides containing the nine-hundred-word description of our model, included separate continuing contributions from the two warring DNA groups at King's. Maurice was later to write that his refusal to publish jointly with the two of us was the biggest mistake of his life.

  Francis an
d I posing with our morning coffee in our office, just after the publication of our manuscript inibiture

  Excerpt from the diary of Gerard Roland Pomerat, of the Rockefeller Foundation, describing his visit to the Cavendish Lab, dated Aprili, 1953

  In every sense solving the double helix was a problem in chemistry. Alex Todd facetiously told me that Francis and I were good organic chemists, not wanting to admit that a major objective in chemistry had been solved by nonchemists. In reality, Francis and I would not have been first to see the structure if Todd's fellow chemists had not done botched jobs. Linus had all the keys to unlock the DNA structure but inexplicably didn't use them that fall of 1952. Rosalind Franklin would have seen the double helix first had she seen fit to enter the model-building race and been better able to interact with other scientists. If she had accepted rather than rejected Maurice as a collaborator, the two of them could not have failed to realize the significance of the monoclinic space group. Dorothy Hodgkin's Oxford put-down of Rosalind as a crystallographer would then not have been the fatal wound that in retrospect it now seems.

  In contrast, Francis and I were far from being on our own. One flight up was the clever Bill Cochran, who put the Bessel functions of helical diffraction theory into Francis's working vocabulary, whence they entered mine. Even more important, Jerry Donohue's spartan desk was no more than twelve feet from mine and Francis's when his quantum chemistry expertise squelched my initial desire to build a double helix based on like-with-like base pairing (e.g., A-A and T-T). The Cavendish was then a magnet for minds that wanted to be challenged by others of equal power. In contrast, Linus Pauling's Caltech was a chemistry garden of mortals hovered over by a god who saw no need to assimilate the ideas and facts of others. If Linus had only spent a few days in Caltech's libraries perusing the literature on DNA that fall, he would most likely had to have hit upon the idea of base pairing and would now be celebrated for both the a-helix and the double helix.

 

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