Unravelling the Double Helix

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Unravelling the Double Helix Page 52

by Gareth Williams


  Figure 20.1 Ray Gosling’s X-ray photograph of ‘crystalline’ DNA (Nobel Foundation)

  Figure 20.2 Francis Crick (Hans Boye/MRC Laboratory of Molecular Biology)

  Figure 20.3 Rosalind Franklin (Elliott & Fry/National Portrait Gallery)

  Figure 21.1 B299: X-ray photograph of DNA, taken by Elwyn Beighton (Special Collections, Leeds Unversity Library)

  Figure 22.1 James D. Watson (Hans Boye/MRC Laboratory of Molecular Biology)

  Figure 24.1 The B form of DNA: Photograph 51 and the X-ray diffraction pattern of squid sperm (King’s College Archives and Nobel Foundation)

  Figure 24.2 Hydrogen bonding linking adenine with thymine and cytosine with guanine, to bridge the two helical strands of DNA (The Double Helix, 1968)

  Figure 24.3 Jim Watson and Francis Crick with their model of the double helix (Science Photo Library)

  Figure 24.4 Schematic drawing of the double helix (The Double Helix, 1968)

  Figure 25.1 Mechanism of duplication of the two strands of DNA (The Double Helix, 1968)

  Figure 25.2 The double helix, refined by Maurice Wilkins (Nobel Foundation)

  Figure 25.3 Francis Crick, Jim Watson and Maurice Wilkins with the other Nobel laureates, 1962 (Science Photo Library)

  Figure 26.1 Colin MacCleod and Maclyn McCarty at the inauguration of the Avery Memorial Gateway, Rockefeller University (Rockefeller University Archives)

  INDEX

  “Page numbers listed correspond to the print edition of this book. You can use your device’s search function to locate particular terms in the text.”

  Page numbers in bold refer to figures.

  adenine

  Kossel’s isolation of 71, 72

  pairing with thymine 248, 324, 338, 339, 340–41

  presence in animal and yeast cells 73, 74

  purine base 75

  structure 76, 76

  Agol, Israel 169, 171

  alkaptonuria 58

  alleles 60

  Alloway, Lionel 150–51

  alpha-helix 253, 291–92, 323

  Altmann, Richard 75

  amino acids 71, 72, 80, 115, 164, 235, 246, 253, 291–92, 353

  ancient DNA 3

  Annals and Magazine of Natural History 23

  antibody research 133–35, 144, 146–48, 149–50, 178–79

  aperiodic crystals 193, 234–35, 267

  Appleton, Edward 227–28

  Arrowsmith (Lewis) 300

  Ascoli, Alfredo 73–74

  Astbury-Bell model of thymonucleic acid 161–62, 162

  Astbury, Dr William (Bill) T.

  background 123

  Cambridge post, failure to get 127

  character 125, 158–59, 378

  conference presentations

  Cold Spring Harbor Conference (1938) 164–65

  Seventh International Genetical Conference (1939) 166–67

  Society for Experimental Biology symposium (1946) 224–25

  Stazione Zoologica conference (1951) 286–87

  Third Conference on Industrial Physics (1939) 165–66

  death 378

  Harvey Lecture 258–59

  keratin’s helical structure 250–51

  Leeds – University

  Beighton’s DNA photograph 288–90, 289

  Department of Biomolecular Structure 223–24

  DNA structure proposal 159–63, 162, 164, 224–25, 235

  letter to Avery 222–23

  move to 128

  MRC grant, disappointment about 233–34

  natural fibres research 155–58

  protein synthesis theory 164–65

  virus research 166–67

  London – Royal Institution

  ‘Astbury-Yardley Tables’ 124

  Bragg’s research group 123–26

  concerns about leaving 154

  human hair X-ray photograph 128, 129, 154–55

  ‘molecular biology,’ invention of term 223, 224, 378–79

  Mozart’s hair 378

  obituary by Bernal 379

  overview of xviii

  photograph 124

  Royal Society Fellowship and Croonian Lecture 223, 224

  Second World War work 196, 223

  ‘Astbury-Yardley Tables’ 124

  Avery, Dr Oswald T.

  accolades

  Gold Medal of the New York Academy of Medicine 218

  Honorary Doctorate from Cambridge 221

  Lasker Award 221

  Nobel Prize nominations 374

  Pasteur Medal 374

  posthumous 375

  Royal Society Copley Medal 221

  Royal Society Foreign Membership 221

  appearance 144–45

  background and education 143–44

  character 145–46, 217–18

  final illness and death 374–75

  influence and legacy 222

  overview of xviii

  photograph 145

  photograph of Griffith 214, 222

  pneumococcal transformation, acceptance of 151, 152

  pneumococcal transformation, scepticism of 148

  retirement 221–22, 373–74

  Rockefeller Institute, New York

  DNA identified as ‘transforming principle’ 206–10

  Burnet’s reaction 215

  Coburn’s reaction 215–16

  J. of Experimental Medicine paper 210–11, 212, 216

  Mirsky’s ridicule of 211, 212, 216–17

  other scientists’ belief in 237–38, 239–40, 301

  reports to Rockefeller Institute 211–12

  lab practices 145

  McCarty’s induction 200, 201

  Member Emeritus status at Rockefeller 210

  move to Rockefeller 144

  partnership with Dochez 146

  pneumococci antibody research 146–48

  ‘Red Seal’ discourses 147–48, 201

  sick leave 148, 149, 151

  single-bacterium culture method 149

  transforming principle research 178, 179–83, 205–10

  Avery, Roy 144, 146, 301, 373

  bacteriophages 219, 300–301

  Baeyer, Adolf 77, 78, 107

  Banks, Sir Joseph 24

  Barcroft, Joseph 204

  Barkla,J.C. 98–99

  base-pairing 6, 6, 8, 247–48, 324–25, 338–39, 339, 340, 347, 372

  bases

  in Astbury-Bell model of thymonucleic acid 160–62, 162, 164, 275

  Chargaff’s Rules 246–48, 247

  in Creeth’s DNA model 260, 261

  in Crick–Watson DNA model 338, 339, 340, 346

  in early Crick–Watson DNA models 309, 337

  families 75

  in Fraser’s DNA model 307

  in Furberg’s DNA model 261, 261, 285

  hydrogen bonding 242, 260, 338

  keto form 338

  Kossel’s research 70–75

  in nucleotide structure 90–91

  number in human genome 4

  in protein synthesis 353

  ratios in DNA 220–21, 235, 246–47, 247, 288–89, 324, 329

  structures 75–76, 76

  in tetranucleotide hypothesis 91, 91

  in Wilkins’ refinement of Crick–Watson DNA model 354

  Bateson, William 58–59, 109–10, 169

  Bathybius haeckelii 27

  Bauer, Franz 26

  Baurhenn, Wilhelm 152

  Bausteine

  of nucleic acids 86, 91

  of nuclein 71–72, 73–74, 78

  of nucleotides 90–91

  of proteins 71, 72, 80, 115; see also amino acids

  Beighton, Elwyn xviii, 196, 224, 288–90, 289, 378

  Bell, Florence 159–63, 162, 164, 165–66, 196, 224

  Benary, Ernst 36–37

  Bernal, John Desmond

  1949 visit to Soviet Union 257

  on Astbury’s character 158–59

  on Astbury’s Leeds post 154–55

  Astbury’s obituary 379
>
  background and education 123

  Cambridge University, Bernal’s lab

  Cambridge Scientists Anti-War Group 190

  lectureship post 127

  protein X-ray crystallography 255

  successes 254

  Wilkins’ disappointment in 189–90

  X-ray diffraction on thymonucleic acid 160

  character 123, 380

  Communism 123, 190, 198, 254, 257, 380–81

  death 381

  Franklin’s obituary 360

  Franklin’s respect for 356

  London – Birkbeck College

  Franklin’s transfer to 321–22

  labs and atmosphere 256

  move to 255

  nucleotide X-ray crystallography 255

  London – Royal Institution 123–26

  obituary by Hodgkin 381

  overview of xviii-xix

  personal life 381

  Royal Society Fellowship 198, 255

  Second World War work 195–96, 255

  Social Function of Science, The (Bernal) 198

  strokes 381

  Vavilov, failure to help 198, 231

  Watson’s book 366

  Bessel, Friedrich 294–95

  Bessel function 295

  biophysics see molecular biology

  Blake, William 371

  Bohr, Niels 114, 176, 301

  Boivin, André 237–38, 244–45, 314

  ‘Boivin-Vendrely Rule’ 244

  Boone, Dr D.W. 69

  Boot, Harry 185, 188, 191, 194, 227

  Borodin, Alexander 81

  Boveri, Theodor 55–56

  Bragg, Robert 98, 102, 103

  Bragg, Sir Lawrence

  accolades

  Barnard Gold Medal 102

  Copley Medal 380

  Military Cross 106

  Nobel Prize 103, 106

  OBE 106

  birth 98

  broken elbow 98

  Cambridge – Cavendish

  Laboratory

  appointed Director 187

  Bragg’s Law 100

  DNA structure research, overseeing

  allowing to restart 330, 335

  Crick and Cochrane’s work on Vand’s theorem 307

  Crick–Watson model, second 340, 348

  Crick–Watson paper for Nature 344

  moratorium on 310

  MRC Unit for the Study of

  the Molecular Structure of

  Biological Systems 272–73

  Pauling’s alpha-helix, reaction to 291–92

  Pauling’s DNA model, reaction to 330

  X-ray crystallography work 99–102

  character 273

  conference presentations

  Pasadena Conference (1953) 352

  Second International Crystallography Congress (1951) 291

  Crick, opinion of 273–74, 371

  death 380

  education 99

  First World War sound-ranging research 102–3

  London – Royal Institution 379

  Manchester – University

  move to 126

  Pauling’s visit 254

  X-ray crystallography work 126

  name 97

  Nature, crystallography paper in 101

  overview of xix

  Patterson function, view of 292

  Pauling, rivalry with 253–54

  photograph 98

  Randall, low opinion of 185

  Watson, opinion of 371

  Watson’s book 364–65

  Watson’s opinion of 303–4

  Wilkins’ Nobel Prize nomination 363–64

  Wilkins’ Royal Society Fellowship 353

  X-Rays and Crystal Structure (Bragg and Bragg) 102

  Bragg, Sir William

  accolades

  Barnard Gold Medal 102

  Nobel Prize 103, 106

  Royal Society Fellowship 99

  Adelaide – University of South

  Australia 97–99

  Astbury, recommending for Leeds post 128

  background and education 97

  Barkla, disputes with 98–99

  character 122, 125–26

  death 196

  family 98

  First World War submarine research 102

  Leeds – University 99–100

  London – Royal Institution

  1919 Christmas Lectures 119

  Astbury’s human hair photograph 128

  crystallography research group 122–26

  Directorship 120, 122–23

  London – University College 106, 120

  name 97

  overview of xix

  photograph 98

  Solvay Conference (1913) 101

  son Bob’s death 103

  X-ray spectrometer, invention of 100

  X-Rays and Crystal Structure (Bragg and Bragg) 102

  Bragg’s Law 100

  BRCA1 gene 1–2, 3–4, 5–7

  Brenner, Sydney 352, 387

  Bridges, Calvin 64, 65, 68, 116, 254

  British Medical Journal 213

  British Society for Social Responsibility in Science 381, 382

  Brockway, Lawrence 252

  Brown, Robert 23–26, 25

  Brownian motion 24

  Burnet, Sir Macfarlane 215, 222

  Carlisle, Harry 257

  Carnegie, Andrew 173

  Carnrick, John 22

  Caspar, Don 357, 358, 359

  Caspersson, Torbjörn 160, 161, 164, 374

  Cavendish, Sir Henry 120

  cell division 28–29, 30–33, 31, 32

  centriole 32, 33

  Chargaff, Erwin

  background 238

  bitterness 364, 377

  character 238–39, 325, 364, 377–78

  Crick, opinion of 325

  death 378

  Gordon Conference (1951) 296

  Gulland’s death 244

  Heraclitean Fire 377

  meeting with Watson and Crick 324–25

  National Medal of Science 377

  New York – Columbia University

  Astbury’s visit 288

  Avery paper’s impact on 222

  Avery’s findings, research confirming 238–40

  Chargaff’s Rules 246–48, 247

  purified DNA gift to Astbury 288–89

  purified DNA gift to Wilkins 296

  tetranucleotide hypothesis, research disproving 245–48, 247

  Wilkins’ visit 296

  Nobel Prize, complaint about not receiving 364

  overview of xix Pasteur Medal 377

  photograph 239

  retirement 377–78

  Watson, opinion of 325, 367, 370

  Chargaff’s Rules 246–48, 247, 324, 325, 329, 334, 338, 342

  Chidgey, Patricia 354–55

  chlorine gas 103–4

  chromatids 32, 34

  chromatin 21, 32, 34–35, 204, 259; see also nuclein

  chromosin 204–5, 216

  chromosomal maps 67–68, 116

  chromosomes

  banding patterns 34

  during cell division 30–33, 31, 32, 160

  chromatin/nuclein as main component 34–35

  duplication 314

  hereditary material 68, 109–10, 116; see also DNA (deoxyribonucleic acid)

  Hertwig’s fertilisation research 53–54

  Morgan’s fruit fly mutation research 63–68, 105, 109–10, 110, 116

  mutations 34, 65–68, 109–10, 110, 116, 225, 230

  numbers 33–34

  sizes and shapes 34

  Sturtevant’s mapping studies 67–68

  Sutton-Boveri theory of inheritance 55–56

  Sutton’s fertilisation research 54–55

  term, invention of 32

  Van Beneden’s fertilisation research 54

  X and Y 67

  Churchill, Winston 102, 272

  Ciba Symposium 357–58

  clupein 165

  Coburn, Alvin
215–16

  Cochrane, Bill 307

  Cold Spring Harbor Symposia

  1938 164–65

  1946 219, 220

  1947

  Boivin’s talk 237–38

  Chargaff’s talk 238–39

  Gulland’s talk 240, 241–43

  Mirsky’s talk 236–37

  proceedings dedicated to Gulland 244

  1953 352

  Cole, Dr Rufus 87, 144, 177

  Conference on Hybridisation and Plant Breeding, Third 58–59

  Conference on Industrial Physics, Third 165–66

  Corey, Robert B. 330

  Cornford, F. M. 373

  Correns, Carl 56, 57

  Coulson, Charles 281

  Creeth, Michael xx, 241–42, 260, 261

  Crick, Francis

  accolades

  Nobel Prize 361, 363, 364

  Royal Society Fellowship 353

  appearance 7

  background 268–69

  Cambridge – Cavendish Laboratory

  Chargaff, meeting with 324

  DNA replication mechanism 351, 351

  DNA structure research

  base-pairing 324–25, 339

  Crick–Watson DNA model, final 7–8, 338–40, 339, 341, 372–73

  Crick–Watson DNA model, first 308–10

  Eagle pub announcement 338, 368

  London–Cambridge Rat Race 330–31, 332, 335

  Nature 25 April 1953 paper 7, 344, 346–47

  Wilkins’ letter from Zurich 325

  Wilkins, talks with 304–5

  X-ray diffraction pattern 307

  genetic code research 353

  move to 271

  protein structure research 310, 323

  transferRNA (tRNA) 353

  virus structure research 352

  Cambridge – Strangeways Research Laboratory 270

  character 273

  death 384

  education 269

  Franklin’s closeness to solving DNA structure 371

  Lawrence Bragg’s dislike of 273–74

  opinions about 273

  overview of xix-xx

  Pauling–Corey DNA model 330

  photograph 269

  relationships

  Franklin 358, 359

  Watson 303, 369

  Wilkins 268, 270, 274–75, 299, 311, 322, 383–84

  San Diego – Salk Institute 383

  Second World War work 269–70

  Watson’s book 364, 365–66, 368

  Watson’s RNA research at Caltech 352

  Crick, Odile (née Speed) 268, 270, 274, 303, 339–40, 344, 358

  Crowfoot, Dorothy see Hodgkin, Dorothy (née Crowfoot)

  Curie, Marie 101

  cytidine 256–57

  cytosine

  Kossel’s isolation of 72

  pairing with guanine 248, 324, 338, 339, 340–41

  presence in animal and yeast cells 73, 74

  pyrimidine base 75

  structure 76, 76

  see also bases

  D-ribose 86, 112, 112, 181–82

  Dale, Sir Henry 221

  Darwin, Charles 46, 50, 52, 60

  Davy, Humphrey 120–21

 

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