Unravelling the Double Helix

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Unravelling the Double Helix Page 54

by Gareth Williams


  Miescher, Friedrich

  Basel – University

  lectureship 14–15

  nuclein research 15

  nutrition studies 16

  overwork 16–17

  professorship 15

  protamine research 15–16

  resignation 18

  salmon studies 15–16

  teaching difficulties 16

  Vesalianum, opening of the 17

  birth and childhood 10

  Davos – sanatorium 17–19, 20–21

  death 19

  heredity, musings on 21

  histochemistry 28

  legacy 19–20

  Leipzig – Ludwig’s laboratory 13–14

  marriage and children 17

  overview of xxii-xxiii

  photograph 18

  tuberculosis 17–19

  Tübingen – Hoppe-Seyler’s

  laboratory joining 11

  leucocyte research 11–12

  nuclei, extraction of 12

  nuclein, discovery of 12–13, 372

  typhus 10

  unfinished work 19–20

  Untersuchungen nuclein paper 14

  Mirsky, Alfred E.

  background 204

  Cold Spring Harbor Conference (1947) 236–37, 238

  Golden Jubilee of Genetics 258

  Harvey Lecture 259

  New York – Rockefeller Institute

  DNA as transforming principle, denial of 211, 212, 216–17, 237, 238, 373

  DNA identified in plasmosin 204–5, 244, 245

  McCarty, feud with 216–17

  not mentioned in Avery’s

  DNA paper 211

  plasmosin/chromosin discovery 203–4, 204–5

  retirement 377

  transforming principle identity research with Avery’s lab 205, 206–7

  overview of xxiii

  photograph 237

  mitosis 30–33, 31, 32

  molecular biology

  building new discipline 234, 270, 385

  invention of term 155, 223, 378–79

  Leeds University’s department 223–24

  Randall’s research proposal 225–26

  Moore, J.E.S. 33

  Morgan, Thomas Hunt

  death 230

  European tour 62–63

  Mechanism of Mendelian Heredity, The (Morgan et al) 68

  Mendel’s research, acceptance of 67–68

  Mendel’s research, mockery of 59, 62–63

  New York – Columbia University

  chromosomal mapping 67–68, 116

  co-inheritance studies 67–68

  departure from 116

  fruit fly mutations research 63–68, 105, 109–10, 110

  gene locations on chromosomes 116

  Nobel Prize 116

  overview of xxiii

  Pasadena – Caltech Institute of Biology 116

  photograph 62

  Royal Society Croonian Lecture 110

  Woods Hole, MA – Marine Biological Laboratory 61–62

  Mudd, Stuart 165

  Muller, Herman 64–65, 66, 68, 114, 169, 171, 218, 230, 300

  mustard gas 105, 179, 199

  mutations 1–2, 3–4, 7, 34, 65–68, 109–10, 110, 116, 225, 230

  Nägeli, Carl von 46–48, 57, 60

  Napp, Abbot Cyrill 39–40, 41, 42, 44, 45, 48

  Nature

  25 April 1953 issue 345–47

  Astbury and Bell, structure of thymonucleic acid 161, 163

  Astbury’s letter about ‘molecular biology’ term 378–79

  Bernal and Crowfoot, pepsin X-Ray crystallography 255

  Bragg (L), halite crystallography 101

  Bragg (L), mica crystallography 101

  Bragg (W), Bragg – Barkla feud 98–99

  Bragg (W), letters about son 101

  Chargaff, base ratios 288

  Crick and Cochrane, protein structure 307

  Crick and Watson, DNA replication mechanism 351

  Crick and Watson, DNA structure 7, 344, 346–47

  Crick and Watson, virus structure 352

  Franklin and Gosling, DNA structure 343, 344, 345–46, 356

  Franklin, TMV 357

  Franklin’s obituary 360

  Furberg, DNA structure 256

  Vavilov’s obituary 230, 231

  Watson, mRNA papers 352

  Wilkins and Gosling, DNA structure 275

  Wilkins, Stokes and Wilson, DNA structure 343–44, 346

  Nature of the Chemical Bond, The (Pauling) 252

  Neufeld, Friedrich 133–35, 140, 141, 142, 149, 175, 229

  New York Times 80, 83, 86, 107, 111, 366–67

  Newton, Margaret 169

  Norrish, Ronald 279–80

  North, Tony 364–65

  Northrop, John 199, 210

  nuclear bomb 192–93, 195, 199

  nuclei, cellular

  Brown’s ‘areola’ studies 25–26, 25

  division 26–27, 28–29

  histological studies 27–28

  Huxley’s denial of importance 27

  ‘one cell, one nucleus’ rule 26

  shapes 26

  term, invention of 26

  nucleic acids

  bases see bases

  building blocks of 86, 91, 91

  as candidate for genetic

  substance 83, 115, 164, 208, 209, 212

  Kossel’s dismissal of 113–14

  Levene’s research 82–83, 85–86, 113

  sugars in 86, 90, 111–12

  term, invention of 75

  viruses 166

  see also DNA (deoxyribonucleic acid); RNA (ribonucleic acid); thymonucleic acid; yeast nucleic acid

  Nucleic Acids (Levene) 113

  nuclein

  building-blocks 70–75

  Flemming’s research 35

  Kossel’s research on bases 70–75

  Miescher’s research 12–15, 21, 28, 69–70

  phosphate content 74

  renamed ‘nucleic acid’ 75

  Russow’s research 34–35

  see also chromatin; nucleic acids

  nucleosides 255–56

  nucleotides

  Chargaff’s Rules 246–48, 247

  name 90–91

  sequence 165, 240, 353

  tetranucleotide hypothesis 91, 91, 112–13, 115, 205, 217, 220–21, 235

  Nunn May, Allen 226–27

  Oliphant, Mark 186, 187–88, 189, 190, 191, 192

  opals 93–94

  Origin of Species, The (Darwin) 52

  Osler, William 87

  Ossietsky, Carl von 175

  paper chromatography 246

  Pasadena Conference 352

  Pasteur, Louis 133

  Path to the Double Helix, The (Olby) 376

  Patterson function/analysis 292, 295–96, 319–20, 345, 356, 357

  Patterson, Lindo 292

  Pauling, Linus

  background and education 251

  Cavendish Laboratory, visits to 254, 344

  Communist sympathies, suspected of 291, 328

  Crick–Watson model, second 344, 347

  Crick’s ‘supercoils’ paper 323

  Langmuir Prize 251

  Nature of the Chemical Bond, The (Pauling) 252

  Nobel Peace Prize 362

  overview of xxiii

  Pasadena – Caltech

  alpha-helix 253, 291

  beta-sheet papers 291

  chemical bonds 252–53

  electron diffraction 352

  Pauling–Corey DNA model 330, 331–32, 372

  Pauling’s Five Rules 251

  Wilkins’ photographs, request for 297

  X-ray crystallography 251

  photograph 252

  PNAS papers 291

  Pauling, Peter 326, 330

  Pavlov, Ivan 81, 107, 111

  Pearson, Karl 59

  Peierls, Rudolf 192

  pentoses 74, 85–86, 90, 112; see also deoxyribose

  pep
sin 35, 181, 255

  Perutz, Max

  background and education 271–72

  Cambridge – Cavendish

  Laboratory

  Crick joining lab 273

  joining 271, 272

  Watson joining lab 303

  Cambridge – University, Bernal’s lab 272

  Franklin’s A-form DNA work 319, 336

  Leeds – University 158

  Nobel Prize 362

  Pauling’s alpha-helix, reaction to 292

  Second World War 272

  Watson’s book 369

  Watson’s imagination 372

  phagocytosis 133–34

  phenotype 59–60

  Planck, Max 95, 96, 106, 107

  plasmosin 203, 204–5

  Ploetz, Alfred 173–74

  Plósz, Pal 14

  pneumonia, lobar

  antibody research 133–35, 146–48

  capsulated vs. non-capsulated strains 133

  description 87, 133

  pneumococci bacteria 133

  Smethwick outbreak 131–32

  ‘soluble specific substance’ (SSS) 146–48, 149, 152, 180–81, 201–2, 206–7

  transformation research 138–42, 140, 149–50, 151

  transforming principle see transforming principle

  treatments 87, 133, 178

  Pollister, Arthur 204, 216, 243, 249

  Pratt, Margaret 318

  Proceedings of the National Academy of Science (PNAS) 291, 301, 330

  protamines 15–16, 72, 80, 114, 204, 235

  Protamines and Histones, The (Kossel) 113–14

  protein synthesis 164, 165, 352–53

  proteins as candidate for genetic substance 21, 114–15, 163–64, 165

  Protonuclein 22, 69, 92

  purines 75–77, 76; see also adenine; base-pairing; guanine

  pus 9–12, 203

  pyrimidines 75–77, 76; see also base-pairing; cytosine; thymine; uracil

  Randall, John T.

  background and education 185

  Birmingham – University

  joining 186

  luminescence research 187

  magnetron, development of the 185

  Wilkins joining lab 186–87

  Cambridge, MA – MIT 227

  Cambridge, UK – Cavendish Laboratory 194

  character 191, 276, 282–83, 287, 383

  Crick – Watson DNA model, final 342

  death 382

  Edinburgh – University 382

  London – General Electric Company 185–86

  London – MRC Biophysics Unit

  applying for MRC grant 296

  camaraderie 276

  cilia research 381–82

  Crick, refusal to offer job to 270

  DNA research, overseeing 296–97, 311, 334

  Franklin, job offer to 277–78, 281–82

  Franklin’s transfer to Birkbeck 321–22

  move to and plans for 228

  Nature 25 April 1953 papers 343

  premises 262–63

  projects 264–65

  team 263–64

  magnetron patent 194, 227

  overview of xxiii-xxiv

  Pasadena Conference 352

  Pauling–Corey DNA model 331

  photograph 186

  Royal Society Fellowship 225, 228

  Royal Society lecture 285

  Royal Society/MRC funding bid 225–26

  ‘Sonnets and Poems’ (Masefield) 386–87

  St Andrews – University 194, 225–26, 228

  Wilkins’s Nobel Prize nomination 361

  Wilkins’s obituary for 382–83

  Wilkins’s opinion of 190

  Wilkins, relationship with 187, 191, 276, 282–83, 287

  Wilkins’ Royal Society

  Fellowship, proposal for 353

  recessive alleles 38, 43, 56, 58, 60

  Recollections from my 50-year career as a bacteriologist (Neufeld) 229

  Reimann, Hobart 141–42

  ribonuclease (RNase) 181–82, 237

  ribose 86, 90, 112, 181–82

  Ris, Hans 236, 244

  Rivers, Tom 177, 178–79, 182, 202, 208–9, 219

  RNA (ribonucleic acid) 3, 181–82, 245, 314, 323, 352, 353, 357, 362; see also yeast nucleic acid

  RNase (ribonuclease) 181–82, 237

  Rockefeller, John D. 83, 86, 173

  Roempler, Louis 36

  Roentgen, Wilhelm 95, 107

  Rous, Peyton 211, 212, 374

  Russow, Edmund 34–35

  Rutherford, Ernest 98, 101, 102, 126, 293

  Salk, Jonas 358

  Sayers, Jim 227

  Schmalhausen, Ivan 53

  Schmidt, W.G. 159

  Schrödinger, Erwin 122, 193, 234–35, 251

  Science 218, 367, 369

  Scott, William 136–37, 213

  Search, The (Snow) 380

  Second World War

  ‘alien’ scientists sent to Canada 272

  Astbury’s war work 196

  Bernal’s war work 195–96

  Leningrad, Siege of 231–32

  Lonsdale’s wartime imprisonment 196

  magnetron, development of the 187–88

  nuclear bomb 192–93, 195

  Poland, Hitler’s invasion of 167

  Rockefeller Institute’s war work 179, 199

  Seeds, Bill 308–9, 334, 364

  Sepia (cuttlefish) 99, 225, 264, 286, 287, 293, 317

  sex-linked traits 67

  Shaw, George Bernard 133–34

  Sia, Richard 149–50

  Signer, Rudolf 160, 266, 316, 317

  Snow, C.P. 366

  Social Function of Science, The (Bernal) 198

  Society for Experimental Biology symposium (1946) 224–25

  sodium thymonucleate see DNA (deoxyribonucleic acid)

  Solvay Conference (1913) 101

  ‘Sonnets and Poems’ (Masefield) 386–87

  Speed, Odile see Crick, Odile (née Speed)

  SSS (soluble specific substance) 146–48, 149, 152, 180–81, 201–2, 206–7

  Stalin, Joseh 170–71, 257

  Stanley, Wendell 210, 359

  Stazione Zoologica conference (1951) 286–87

  Stokes, Alec 264–65, 282, 294–95, 307, 308–9, 334, 344, 345, 346, 364

  Strangeways, Thomas 270–71

  Studies of Nuclein and its Breakdown Products (Kossel) 71

  Studies of plant hybridisation (Mendel) 46–47, 49–50, 56, 57–58

  Sturtevant, Alfred 64, 67–68, 116, 254

  sulfanilamide 200

  sulfapyridine 178

  Summers, Dr T.O. 22

  Sutton-Boveri chromosomal theory of inheritance 55–56, 105

  Sutton, Walter xxiv, 54–56, 105

  Synge, Richard 246

  Taylor, Harriett F.W. 219, 220, 222, 241

  tetranucleotide hypothesis 91, 91, 112–13, 112, 115, 205, 217, 220–21, 235, 241, 247

  Third Man of the Double Helix, The (Wilkins) 384

  Thomson, J.J. 99, 100, 121

  Threlfall, J.C. 241

  thymine

  absence from yeast cells 73, 74

  Kossel’s isolation of 72

  pairing with adenine 248, 324, 338, 339, 340–41

  presence in animal cells 74

  pyrimidine base 75

  structure 76, 76

  see also bases

  thymonucleic acid 75, 159–63, 162, 164–65

  thymus 26, 72

  timeline xiii-xvii

  Times 107, 360

  titrimeter 199

  Tizard, Sir Henry 184–85

  tobacco mosaic virus (TMV) 310–11, 323, 356–57, 359, 360

  ‘Transformation of pneumococcal types in vitro, The’ (Dawson and Sia) 150

  transforming principle

  components 182

  identified as DNA 204–9

  Astbury’s reaction 222–23

  Avery, MacLeod and McCarty’s research 205–10


  Avery’s report to Institute 211–12

  Boivin’s confirmatory research 237

  Hotchkiss’ confirmatory research 220–21

  J. of Experimental Medicine paper 210–11, 212, 216, 222

  Mirsky’s ridicule of 216–17

  Taylor’s confirmatory research 220

  identity research

  Alloway’s 151

  Avery and MacLeod’s 177–78, 180–82

  Avery and McCarty’s 201–2

  Dawson and Sia’s 150

  Dawson’s 149

  properties 152

  trypsin 181

  Tschermak, Erich von 57

  tuberculosis research 17–19, 135, 136

  uracil 73–74, 75, 76

  Van Beneden, Edouard 29, 33, 54

  Van Slyke, Donald 85, 88

  Vand, Vladimir 307

  Vavilov, Nikolai

  appearance and character 169

  arrest 171–72

  ascent under Lenin 170

  death in prison 231

  disappearance 171–72, 184, 197

  imprisonment 230–31, 231

  International Conference on Genetics (1939) 166

  Lysenko’s hatred of 170, 171

  overview of xxiv

  photographs 169, 231

  Royal Society Foreign Membership 197–98

  scientific convictions 171

  seed bank 168–69, 231–32

  wheat genetics 168–69

  Vegard, Lars 99

  Vendrely, Roger and Colette 244

  Vesalius, Andreas 17

  virus research

  bacteriophages 219, 300–301, 302

  and nucleic acids 166–67

  poliovirus 358

  tobacco mosaic virus (TMV) 310–11, 323, 356–57, 359, 360

  Vischer, Ernst 246–48

  Waldeyer, Wilhelm 32

  Watson, Elizabeth 344

  Watson, James (Jim) D.

  2005 public lecture 385

  background 300

  California – Caltech 352

  Cambridge, MA – Harvard University 352

  Cambridge, UK – Cavendish

  Laboratory

  Chargaff, meeting with 324

  Crick–Watson DNA model, final 7–8, 335–39, 339, 340, 341, 372–73

  Crick–Watson DNA model, initial 308–10

  DNA base-pairing 338

  DNA hydrogen bonds 337–38

  DNA Photograph 51, being shown 328–29, 332–34

  DNA replication mechanism 351, 351

  Hardy Club talk on DNA model 349

  joining 299–300, 302–3

  London–Cambridge Rat Race 330–31, 332, 335

  MRC Biophysics Unit colloquium 1951 305–6

  opinions about people and place 303–4

  talks with Wilkins and Crick 304–5

  tobacco mosaic virus (TMV) research 310–11, 323

  virus structure research 352

  Wilkins’ letter from Zurich 325

  character 300, 385–86

  conference presentations and attendances

  Cold Spring Harbor Symposium (1953) 352

  Pasadena Conference on protein structure (1953) 352

  Stazione Zoologica Conference (1951) 299, 302

 

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